Hotspot

ndhC-trnV-UAC

LSC · 49784-50946 · 1163 bp

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Pi 0.0147

MarkerSeek score 54.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 17
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.9	57.8	1219-1262 bp	1.0	41.3
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.9	57.5	1218-1261 bp	1.0	41.3
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	1220-1263 bp	1.0	41.3
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	1219-1262 bp	1.0	41.3
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	1207-1250 bp	1.0	41.3