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psbZ-trnG-GCC

LSC · 35655-35973 · 319 bp

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Pi 0.0285

MarkerSeek score 63.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 319
variable_sites: 9
pi: 0.0285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	476-480 bp	1.0	79.0
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	474-478 bp	1.0	78.6
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	ATGACTTCATTTTGGGCGGA	50.0	45.0	57.8	57.8	514-518 bp	1.0	78.5
psbZ-trnG-GCC_p4	TTGCTTCTCCTGATGGTTGG	TGACTTCATTTTGGGCGGAT	50.0	45.0	57.8	57.8	513-517 bp	1.0	78.5
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	TGACTTCATTTTGGGCGGAT	55.0	45.0	58.3	57.8	511-515 bp	1.0	78.1