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trnC-GCA-petN

LSC · 9300-10134 · 835 bp

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Pi 0.0135

MarkerSeek score 67.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 11
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTATATCGTTTTGGCGGCA	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.0	58.2	924-946 bp	1.0	81.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	869-891 bp	1.0	81.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	887-909 bp	1.0	78.9
trnC-GCA-petN_p4	TGTATATCGTTTTGGCGGCA	GCCCAAGCGAGACTTACTAT	45.0	50.0	58.0	57.1	923-945 bp	1.0	75.5
trnC-GCA-petN_p5	TGTATATCGTTTTGGCGGCA	ATTAAAGCAGCCCAAGCGAG	45.0	50.0	58.0	58.9	932-954 bp	1.0	75.4