Hotspot

trnS-GCU-trnC-GCA

LSC · 8482-9228 · 747 bp

Back to Tagetes

Pi 0.0122
MarkerSeek score 61.20
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
747
variable_sites
9
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 ACGGAAAGAGAGGGATTCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 898 bp 1.0 79.8
trnS-GCU-trnC-GCA_p2 ACGGAAAGAGAGGGATTCGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 880 bp 1.0 77.7
trnS-GCU-trnC-GCA_p3 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 834 bp 1.0 76.1
trnS-GCU-trnC-GCA_p4 AACGGAAAGAGAGGGATTCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.3 58.1 899 bp 1.0 75.6
trnS-GCU-trnC-GCA_p5 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 816 bp 1.0 73.9