Hotspot

rpl16

LSC · 78831-80555 · 1725 bp

Back to Taeniophyllum

Pi 0.107

MarkerSeek score 81.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1725
variable_sites: 168
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	ACGGAAAAGAGATTGCACGT	45.0	45.0	58.0	58.1	2210-2320 bp	1.0	90.2
rpl16_p2	TTGGGCACTGCTTCTTTGAT	ACGGAAAAGAGATTGCACGT	45.0	45.0	58.0	58.1	2211-2321 bp	1.0	90.2
rpl16_p3	TGGGCACTGCTTCTTTGATT	GAGAAGGCAGAGTTCCCCTA	45.0	55.0	58.0	58.1	2177-2287 bp	1.0	90.2
rpl16_p4	TTGGGCACTGCTTCTTTGAT	GAGAAGGCAGAGTTCCCCTA	45.0	55.0	58.0	58.1	2178-2288 bp	1.0	90.2
rpl16_p5	TGGGCACTGCTTCTTTGATT	AAGAGATTGCACGTGTCGAA	45.0	45.0	58.0	57.9	2204-2314 bp	1.0	90.0