Hotspot

ccsA-psaC

SSC · 109212-110353 · 1142 bp

Back to Taeniophyllum

Pi 0.0628

MarkerSeek score 58.30

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1142
variable_sites: 13
pi: 0.0628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.18
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	560-1488 bp	1.0	84.7
ccsA-psaC_p2	AGGCATTCTTTCTGGAGCAG	GCTTGTCCCACGGATGTATT	50.0	50.0	57.9	58.0	659-1587 bp	1.0	84.7
ccsA-psaC_p3	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.2	58.0	559-1487 bp	1.0	84.4
ccsA-psaC_p4	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	550-1478 bp	1.0	84.3
ccsA-psaC_p5	TTCTGGAGCAGTATGGGCTA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	551-1479 bp	1.0	84.3