Hotspot

rpl32-trnL-UAG

SSC · 107521-108041 · 521 bp

Back to Taeniophyllum

Pi 0.133

MarkerSeek score 85.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 521
variable_sites: 59
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	620-648 bp	1.0	90.1
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	613-641 bp	1.0	89.7
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	619-647 bp	1.0	89.6
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	618-646 bp	1.0	89.6
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	614-642 bp	1.0	89.0