Hotspot

trnN-GUU-rpl32

IRb · 105937-107346 · 1410 bp

Back to Taeniophyllum

Pi 0.11

MarkerSeek score 70.80

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: IRb
length_bp: 1410
variable_sites: 92
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.59
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1133-1664 bp	1.0	88.7
trnN-GUU-rpl32_p2	TATGCCGAGGTATTGAGGGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	57.9	58.0	1243-1775 bp	1.0	88.3
trnN-GUU-rpl32_p3	ATCCAGTATAGACGGGGTGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	58.0	58.0	1723-2221 bp	1.0	88.2
trnN-GUU-rpl32_p4	ATCCAGTATAGACGGGGTGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	2351-2821 bp	1.0	88.2
trnN-GUU-rpl32_p5	CGGCCGCAAAGATATTGAAC	CTTTTTGACTGTCCGGTGGA	50.0	50.0	57.9	58.0	1575-2067 bp	1.0	87.7