Hotspot

psaJ-rpl33

LSC · 63801-64268 · 468 bp

Back to Taeniophyllum

Pi 0.13

MarkerSeek score 82.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 58
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AAAACAAACGCGCTACCAAG	TGAGCTTGAGTTCCGATTGG	45.0	50.0	57.9	57.9	1752-1790 bp	1.0	88.0
psaJ-rpl33_p2	CAAAACAAACGCGCTACCAA	TGAGCTTGAGTTCCGATTGG	45.0	50.0	58.2	57.9	1753-1791 bp	1.0	87.5
psaJ-rpl33_p3	GATGCCTTGTCATTCCCCTT	TGAGCTTGAGTTCCGATTGG	50.0	50.0	57.9	57.9	1230-1242 bp	1.0	86.1
psaJ-rpl33_p4	AGTGGGCGACAAAATGGAAT	TGAGCTTGAGTTCCGATTGG	45.0	50.0	58.1	57.9	1576 bp	0.5	58.6
psaJ-rpl33_p5	GGCGACAAAATGGAATCTGC	TGAGCTTGAGTTCCGATTGG	50.0	50.0	58.1	57.9	1572 bp	0.5	58.5