Hotspot

trnP-UGG-psaJ

LSC · 63243-63665 · 423 bp

Back to Taeniophyllum

Pi 0.0926
MarkerSeek score 82.90
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
423
variable_sites
34
pi
0.0926
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG CCTGCTAAAGACCCAAACCA 45.0 50.0 57.9 57.7 479-529 bp 1.0 86.2
trnP-UGG-psaJ_p2 AAAACAAACGCGCTACCAAG ACCTGCTAAAGACCCAAACC 45.0 50.0 57.9 57.7 480-530 bp 1.0 86.2
trnP-UGG-psaJ_p3 CAAAACAAACGCGCTACCAA CCTGCTAAAGACCCAAACCA 45.0 50.0 58.2 57.7 480-530 bp 1.0 85.7
trnP-UGG-psaJ_p4 CAAAACAAACGCGCTACCAA ACCTGCTAAAGACCCAAACC 45.0 50.0 58.2 57.7 481-531 bp 1.0 85.7
trnP-UGG-psaJ_p5 TTGTACCCAAAACAAACGCG CCTGCTAAAGACCCAAACCA 45.0 50.0 57.8 57.7 487-537 bp 1.0 85.5