Hotspot

petA-psbJ

LSC · 59494-60468 · 975 bp

Back to Taeniophyllum

Pi 0.106

MarkerSeek score 82.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 99
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.0	58.0	1080-1084 bp	1.0	90.0
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CGGTTCCTATTCTGGGTTGG	50.0	55.0	58.0	58.0	1094 bp	0.5	59.7
petA-psbJ_p3	ATTAGCCCCACTCGATCGTA	CGGTTCCTATTCTGGGTTGG	50.0	55.0	58.0	58.0	1622 bp	0.5	59.6
petA-psbJ_p4	TAGCCCCACTCGATCGTATT	CGGTTCCTATTCTGGGTTGG	50.0	55.0	58.0	58.0	1620 bp	0.5	59.6
petA-psbJ_p5	TTAGCCCCACTCGATCGTAT	CGGTTCCTATTCTGGGTTGG	50.0	55.0	58.0	58.0	1621 bp	0.5	59.6