Hotspot

trnF-GAA-trnV-UAC

LSC · 47067-47705 · 639 bp

Back to Taeniophyllum

Pi 0.17

MarkerSeek score 85.60

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 98
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	706-1171 bp	1.0	90.0
trnF-GAA-trnV-UAC_p2	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	749-1214 bp	1.0	88.8
trnF-GAA-trnV-UAC_p3	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	748-1213 bp	1.0	88.8
trnF-GAA-trnV-UAC_p4	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	705-1170 bp	1.0	87.3
trnF-GAA-trnV-UAC_p5	TCGGGATAGCTCAGTTGGTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	744-1209 bp	1.0	86.8