Hotspot

psbM-trnD-GUC

LSC · 28564-29607 · 1044 bp

Back to Taeniophyllum

Pi 0.0968

MarkerSeek score 83.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1044
variable_sites: 95
pi: 0.0968
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAGAATTCGAGGAGTTGGGC	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1700 bp	0.5	58.5
psbM-trnD-GUC_p2	GAATTCGAGGAGTTGGGCTT	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1698 bp	0.5	58.5
psbM-trnD-GUC_p3	AAGAATTCGAGGAGTTGGGC	GTATGATGGCAGTCTAGGCG	50.0	55.0	57.9	57.9	2167 bp	0.5	58.5
psbM-trnD-GUC_p4	GAATTCGAGGAGTTGGGCTT	GTATGATGGCAGTCTAGGCG	50.0	55.0	57.9	57.9	2165 bp	0.5	58.5
psbM-trnD-GUC_p5	GAATTCGAGGAGTTGGGCTT	GGAAAGACTCCCCGGATCTA	50.0	55.0	57.9	57.9	2239 bp	0.5	58.4