Hotspot

trnS-GCU-trnG-GCC

LSC · 8037-8675 · 639 bp

Back to Taeniophyllum

Pi 0.132

MarkerSeek score 87.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 80
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	708-1196 bp	1.0	61.1
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	707-1195 bp	1.0	61.1
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	711-1199 bp	1.0	60.0
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	710-1198 bp	1.0	60.0
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	709-1197 bp	1.0	60.0