Hotspot

psbK-psbI

LSC · 7229-7675 · 447 bp

Back to Taeniophyllum

Pi 0.106

MarkerSeek score 82.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 44
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCGTTAGATAAG	50.0	55.0	57.9	57.6	565-615 bp	1.0	86.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	585-635 bp	1.0	86.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	562-612 bp	1.0	84.6
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	563-613 bp	1.0	84.6
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	564-614 bp	1.0	84.6