Hotspot

rps16-trnQ-UUG

LSC · 5776-6591 · 816 bp

Back to Taeniophyllum

Pi 0.152
MarkerSeek score 87.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
816
variable_sites
120
pi
0.152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 912-972 bp 1.0 89.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 889-949 bp 1.0 88.8
rps16-trnQ-UUG_p3 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 910-970 bp 1.0 88.6
rps16-trnQ-UUG_p4 ATCCACAGCCAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 916-976 bp 1.0 88.3
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 900-960 bp 1.0 88.3