Hotspot

matK-trnK-UUU

LSC · 3171-4088 · 918 bp

Back to Taeniophyllum

Pi 0.102

MarkerSeek score 82.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 91
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGGAACATCTTTGAGCCAGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.2	58.2	1491-1688 bp	1.0	85.0
matK-trnK-UUU_p2	AGGAACATCTTTGAGCCAGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.2	58.2	1490-1687 bp	1.0	85.0
matK-trnK-UUU_p3	AGGAACATCTTTGAGCCAGC	TAGATGTTCGATCCCGACGA	50.0	50.0	58.2	58.0	2136 bp	0.5	58.3
matK-trnK-UUU_p4	TTCTTGTGCAGCGAAAATGC	TAGATGTTCGATCCCGACGA	45.0	50.0	58.2	58.0	1871 bp	0.5	57.8
matK-trnK-UUU_p5	TTTGAGCCAGCATTGAAGGA	TAGATGTTCGATCCCGACGA	45.0	50.0	57.7	58.0	2127 bp	0.5	57.3