Pi
0.00067
MarkerSeek score
59.10
Primer available
yes
Label
rrn16
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rrn16_p1 | TGGCGAACTCCAGGCTAATA | CAGACGTAGCTGCCTTCTTT | 50.0 | 50.0 | 58.5 | 57.9 | 1690 bp | 1.0 | 75.3 |
| rrn16_p2 | GGCGAACTCCAGGCTAATAA | CAGACGTAGCTGCCTTCTTT | 50.0 | 50.0 | 57.4 | 57.9 | 1689 bp | 1.0 | 74.4 |
| rrn16_p3 | TGGCGAACTCCAGGCTAATA | TTTTGGGCGTGAAGAAGTGT | 50.0 | 45.0 | 58.5 | 58.2 | 1672 bp | 1.0 | 74.0 |
| rrn16_p4 | GGCGAACTCCAGGCTAATAA | TTTTGGGCGTGAAGAAGTGT | 50.0 | 45.0 | 57.4 | 58.2 | 1671 bp | 1.0 | 73.2 |
| rrn16_p5 | TGGCGAACTCCAGGCTAATA | GCGTGAAGAAGTGTCAAACC | 50.0 | 50.0 | 58.5 | 57.6 | 1666 bp | 1.0 | 72.9 |
| rrn16_p1 | CAGACGTAGCTGCCTTCTTT | TGGCGAACTCCAGGCTAATA | 50.0 | 50.0 | 57.9 | 58.5 | 1690 bp | 1.0 | 75.3 |
| rrn16_p2 | CAGACGTAGCTGCCTTCTTT | GGCGAACTCCAGGCTAATAA | 50.0 | 50.0 | 57.9 | 57.4 | 1689 bp | 1.0 | 74.4 |
| rrn16_p3 | TTTTGGGCGTGAAGAAGTGT | TGGCGAACTCCAGGCTAATA | 45.0 | 50.0 | 58.2 | 58.5 | 1672 bp | 1.0 | 74.0 |
| rrn16_p4 | TTTTGGGCGTGAAGAAGTGT | GGCGAACTCCAGGCTAATAA | 45.0 | 50.0 | 58.2 | 57.4 | 1671 bp | 1.0 | 73.2 |
| rrn16_p5 | GCGTGAAGAAGTGTCAAACC | TGGCGAACTCCAGGCTAATA | 50.0 | 50.0 | 57.6 | 58.5 | 1666 bp | 1.0 | 72.9 |