Hotspot

psbD

LSC · 8638-9699 · 1062 bp

Back to Taeniatherum

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AGTGATATGAGGTGCTCGGA	GTCACGACCAGCTAAAACGA	50.0	50.0	57.9	58.2	1142 bp	1.0	77.7
psbD_p2	TGCTCGGAAATGGTTGAAGT	GTCACGACCAGCTAAAACGA	45.0	50.0	57.7	58.2	1130 bp	1.0	76.1
psbD_p3	AGTGATATGAGGTGCTCGGA	TCACGACCAGCTAAAACGAA	50.0	45.0	57.9	57.5	1141 bp	1.0	75.4
psbD_p4	TGCTCGGAAATGGTTGAAGT	TCACGACCAGCTAAAACGAA	45.0	45.0	57.7	57.5	1129 bp	1.0	73.8
psbD_p5	GTGCTCGGAAATGGTTGAAG	GTCACGACCAGCTAAAACGA	50.0	50.0	57.4	58.2	1131 bp	1.0	73.3