Hotspot

trnN-GUU-rps15

IRb · 100379-101909 · 1531 bp

Back to Taeniatherum

Pi 0.00131
MarkerSeek score 59.40
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
IRb
length_bp
1531
variable_sites
2
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 ACGACCAGTCAGTTAACAGC CAGAACCCCTGTTTTCTTCT 50.0 45.0 57.8 55.2 1653 bp 1.0 56.2
trnN-GUU-rps15_p2 AGGATTCGAACCTACGACCA CAGAACCCCTGTTTTCTTCT 50.0 45.0 58.2 55.2 1666 bp 1.0 56.2
trnN-GUU-rps15_p3 GGATTCGAACCTACGACCAG CAGAACCCCTGTTTTCTTCT 55.0 45.0 57.8 55.2 1665 bp 1.0 55.8
trnN-GUU-rps15_p4 ACGACCAGTCAGTTAACAGC ACTTGAAATTCAACAGAACCCC 50.0 40.9 57.8 57.1 1666 bp 1.0 55.4
trnN-GUU-rps15_p5 AGGATTCGAACCTACGACCA ACTTGAAATTCAACAGAACCCC 50.0 40.9 58.2 57.1 1679 bp 1.0 55.3