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psbE-petL

LSC · 62775-63946 · 1172 bp

Back to Taeniatherum

Pi 0.000853

MarkerSeek score 59.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 1
pi: 0.000853
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	45.0	50.0	58.0	58.3	1376 bp	1.0	77.8
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	50.0	50.0	57.9	58.3	1365 bp	1.0	77.6
psbE-petL_p3	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	50.0	50.0	58.2	58.3	1367 bp	1.0	76.1
psbE-petL_p4	TGACAAATAACCAACCCGCA	GGCGTATCTTGTTCAAGCCA	45.0	50.0	58.0	58.6	1375 bp	1.0	75.5
psbE-petL_p5	CAACCCGCAATGAATAGGGA	GGCGTATCTTGTTCAAGCCA	50.0	50.0	57.9	58.6	1364 bp	1.0	75.2