Hotspot

rps16-trnQ-UUG

LSC · 5556-6304 · 749 bp

Back to Taeniatherum

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
749
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 822 bp 1.0 78.4
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 833 bp 1.0 77.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AGTGTCGCATAAAACCCGAA 45.0 45.0 58.1 57.8 891 bp 1.0 77.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 826 bp 1.0 77.3
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC AGTGTCGCATAAAACCCGAA 50.0 45.0 58.2 57.8 902 bp 1.0 77.1