primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5556	6304	822-822	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5556	6304	833-833	1.000000	77.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	20	20	45.0	45.0	58.1	57.8	8.1	0.0	0.312175	5556	6304	891-891	1.000000	77.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5556	6304	826-826	1.000000	77.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.377084	5556	6304	902-902	1.000000	77.1
psbD_p1	psbD	1	AGTGATATGAGGTGCTCGGA	GTCACGACCAGCTAAAACGA	20	20	50.0	50.0	57.9	58.2	0.0	8.3	0.298614	8638	9699	1142-1142	1.000000	77.7
psbD_p2	psbD	2	TGCTCGGAAATGGTTGAAGT	GTCACGACCAGCTAAAACGA	20	20	45.0	50.0	57.7	58.2	0.0	8.3	0.495713	8638	9699	1130-1130	1.000000	76.1
psbD_p3	psbD	3	AGTGATATGAGGTGCTCGGA	TCACGACCAGCTAAAACGAA	20	20	50.0	45.0	57.9	57.5	0.0	8.3	0.585737	8638	9699	1141-1141	1.000000	75.4
psbD_p4	psbD	4	TGCTCGGAAATGGTTGAAGT	TCACGACCAGCTAAAACGAA	20	20	45.0	45.0	57.7	57.5	0.0	8.3	0.782837	8638	9699	1129-1129	1.000000	73.8
psbD_p5	psbD	5	GTGCTCGGAAATGGTTGAAG	GTCACGACCAGCTAAAACGA	20	20	50.0	50.0	57.4	58.2	0.0	8.3	0.844375	8638	9699	1131-1131	1.000000	73.3
psaA-ycf3_p1	psaA-ycf3	1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.091171	41422	42096	836-836	1.000000	79.4
psaA-ycf3_p2	psaA-ycf3	2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.132613	41422	42096	832-832	1.000000	79.0
psaA-ycf3_p3	psaA-ycf3	3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	57.8	58.0	0.0	18.7	0.235180	41422	42096	834-834	1.000000	78.2
psaA-ycf3_p4	psaA-ycf3	4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	57.8	58.0	0.0	18.7	0.235180	41422	42096	835-835	1.000000	78.2
psaA-ycf3_p5	psaA-ycf3	5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.276621	41422	42096	830-830	1.000000	77.9
rbcL-psaI_p1	rbcL-psaI	1	CGCAGCTTGTGAAGTATGGA	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.3	57.9	0.0	26.9	0.351772	56147	57814	1803-1803	1.000000	77.3
rbcL-psaI_p2	rbcL-psaI	2	AGTTCGAGCCGGTAGATACT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.7	57.9	1.9	26.9	0.422537	56147	57814	1768-1768	1.000000	76.7
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.6	57.9	0.0	26.9	0.430670	56147	57814	1782-1782	1.000000	76.6
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	57.6	57.9	0.0	26.9	0.472342	56147	57814	1787-1787	1.000000	76.3
rbcL-psaI_p5	rbcL-psaI	5	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.5	57.9	11.3	26.9	0.534920	56147	57814	1830-1830	1.000000	75.8
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.895979	61180	62005	1015-1020	1.000000	73.2
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.909471	61180	62005	998-1003	1.000000	73.1
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	58.2	57.1	13.8	0.0	1.036375	61180	62005	1012-1017	1.000000	72.1
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	ACTGTAGCTGGTATTGCTGTG	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.060473	61180	62005	1021-1026	1.000000	71.9
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	ACTGTAGCTGGTATTGCTGTG	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.073965	61180	62005	1004-1009	1.000000	71.8
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	20	20	45.0	50.0	58.0	58.3	0.0	7.6	0.278273	62775	63946	1376-1376	1.000000	77.8
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	57.9	58.3	6.4	7.6	0.312268	62775	63946	1365-1365	1.000000	77.6
psbE-petL_p3	psbE-petL	3	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	58.2	58.3	0.0	7.6	0.498226	62775	63946	1367-1367	1.000000	76.1
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	GGCGTATCTTGTTCAAGCCA	20	20	45.0	50.0	58.0	58.6	0.0	7.6	0.568355	62775	63946	1375-1375	1.000000	75.5
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	GGCGTATCTTGTTCAAGCCA	20	20	50.0	50.0	57.9	58.6	6.4	7.6	0.602350	62775	63946	1364-1364	1.000000	75.2
infA-rps8_p1	infA-rps8	1	AACATTCCATTGGGTAGGGC	GGGTGGAATGGGAATTGCTA	20	20	50.0	50.0	57.8	57.5	2.1	0.0	0.611780	77013	77092	284-284	1.000000	75.4
infA-rps8_p2	infA-rps8	2	GGGTAGGGCTTCCGTAACTA	GGGTGGAATGGGAATTGCTA	20	20	55.0	50.0	58.2	57.5	1.4	0.0	0.672098	77013	77092	273-273	1.000000	74.9
infA-rps8_p3	infA-rps8	3	ATTGGGTAGGGCTTCCGTAA	GGGTGGAATGGGAATTGCTA	20	20	50.0	50.0	58.4	57.5	0.0	0.0	0.865534	77013	77092	276-276	1.000000	73.4
infA-rps8_p4	infA-rps8	4	AACATTCCATTGGGTAGGGC	TTGGGTGGAATGGGAATTGC	20	20	50.0	50.0	57.8	58.7	2.1	0.0	0.884467	77013	77092	286-286	1.000000	73.2
infA-rps8_p5	infA-rps8	5	CATTCCATTGGGTAGGGCTT	GGGTGGAATGGGAATTGCTA	20	20	50.0	50.0	57.5	57.5	2.1	0.0	0.912156	77013	77092	282-282	1.000000	73.0
trnI-CAU-trnL-CAA_p1	trnI-CAU-trnL-CAA	1	GTTGGGCGCTTTAACCATTC	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	7.6	15.9	0.017031	83608	85991	2480-2485	1.000000	80.1
trnI-CAU-trnL-CAA_p2	trnI-CAU-trnL-CAA	2	GTTGGGCGCTTTAACCATTC	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	7.6	15.9	0.018402	83608	85991	2482-2487	1.000000	80.0
trnI-CAU-trnL-CAA_p3	trnI-CAU-trnL-CAA	3	GTTGGGCGCTTTAACCATTC	TGGTGAAATGGTAGACACGC	20	20	50.0	50.0	58.0	58.2	7.6	0.0	0.205867	83608	85991	2485-2490	1.000000	78.5
trnI-CAU-trnL-CAA_p4	trnI-CAU-trnL-CAA	4	GTTGGGCGCTTTAACCATTC	GAAATGGTAGACACGCGAGA	20	20	50.0	50.0	58.0	57.7	7.6	15.9	0.282570	83608	85991	2481-2486	1.000000	77.9
trnI-CAU-trnL-CAA_p5	trnI-CAU-trnL-CAA	5	GTTGGGCGCTTTAACCATTC	GTTCGAGTCCTCTTCAAGGC	20	20	50.0	55.0	58.0	58.3	7.6	0.0	0.293208	83608	85991	2432-2437	1.000000	77.9
ndhB-rps7_p1	ndhB-rps7	1	AGAGAAGCAAATGAAACGCT	ATGGCAGAGGCAAATAGAGC	20	20	40.0	50.0	56.0	58.0	0.0	0.0	2.059145	88890	89188	417-417	1.000000	63.9
ndhB-rps7_p2	ndhB-rps7	2	TGAAGAGAAGCAAATGAAACGC	ATGGCAGAGGCAAATAGAGC	22	20	40.9	50.0	58.1	58.0	0.0	0.0	2.132612	88890	89188	420-420	1.000000	63.3
ndhB-rps7_p3	ndhB-rps7	3	GAAGAGAAGCAAATGAAACGCT	ATGGCAGAGGCAAATAGAGC	22	20	40.9	50.0	57.8	58.0	0.0	0.0	2.174152	88890	89188	419-419	1.000000	63.0
ndhB-rps7_p4	ndhB-rps7	4	AAGAGAAGCAAATGAAACGCT	ATGGCAGAGGCAAATAGAGC	21	20	38.1	50.0	56.7	58.0	0.0	0.0	2.293459	88890	89188	418-418	1.000000	62.0
ndhB-rps7_p5	ndhB-rps7	5	AGAGAAGCAAATGAAACGCT	GTAGATGCTGCCAAAGGGAG	20	20	40.0	55.0	56.0	58.3	0.0	0.0	2.364431	88890	89188	474-474	1.000000	61.4
rrn16_p1	rrn16	1	TGGCGAACTCCAGGCTAATA	CAGACGTAGCTGCCTTCTTT	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.594386	92535	94026	1690-1690	1.000000	75.3
rrn16_p2	rrn16	2	GGCGAACTCCAGGCTAATAA	CAGACGTAGCTGCCTTCTTT	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.700883	92535	94026	1689-1689	1.000000	74.4
rrn16_p3	rrn16	3	TGGCGAACTCCAGGCTAATA	TTTTGGGCGTGAAGAAGTGT	20	20	50.0	45.0	58.5	58.2	0.0	0.0	0.750649	92535	94026	1672-1672	1.000000	74.0
rrn16_p4	rrn16	4	GGCGAACTCCAGGCTAATAA	TTTTGGGCGTGAAGAAGTGT	20	20	50.0	45.0	57.4	58.2	0.0	0.0	0.857146	92535	94026	1671-1671	1.000000	73.2
rrn16_p5	rrn16	5	TGGCGAACTCCAGGCTAATA	GCGTGAAGAAGTGTCAAACC	20	20	50.0	50.0	58.5	57.6	0.0	0.0	0.893230	92535	94026	1666-1666	1.000000	72.9
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	ACGACCAGTCAGTTAACAGC	CAGAACCCCTGTTTTCTTCT	20	20	50.0	45.0	57.8	55.2	8.6	0.0	2.982252	100379	101909	1653-1653	1.000000	56.2
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	AGGATTCGAACCTACGACCA	CAGAACCCCTGTTTTCTTCT	20	20	50.0	45.0	58.2	55.2	15.0	0.0	2.988487	100379	101909	1666-1666	1.000000	56.2
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	GGATTCGAACCTACGACCAG	CAGAACCCCTGTTTTCTTCT	20	20	55.0	45.0	57.8	55.2	0.0	0.0	3.037162	100379	101909	1665-1665	1.000000	55.8
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	ACGACCAGTCAGTTAACAGC	ACTTGAAATTCAACAGAACCCC	20	22	50.0	40.9	57.8	57.1	8.6	4.0	3.087598	100379	101909	1666-1666	1.000000	55.4
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	AGGATTCGAACCTACGACCA	ACTTGAAATTCAACAGAACCCC	20	22	50.0	40.9	58.2	57.1	15.0	4.0	3.093833	100379	101909	1679-1679	1.000000	55.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAGCGGTAATGAGCATCCAA	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050149	105900	106609	845-846	1.000000	79.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTTTCGAGCGGTAATGAGCA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.091209	105900	106609	850-851	1.000000	79.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAGCGGTAATGAGCATCCAA	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105116	105900	106609	848-849	1.000000	79.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	105900	106609	820-821	1.000000	79.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	105900	106609	819-820	1.000000	79.1
ndhH_p1	ndhH	1	AGGAGGGTCAAAATGGGTAGA	TTTGATGAGCCTCGCTTTGA	21	20	47.6	45.0	58.1	57.8	0.0	0.0	1.254177	114301	115482	1364-1364	1.000000	70.0
ndhH_p2	ndhH	2	AAAGGAGGGTCAAAATGGGT	TTTGATGAGCCTCGCTTTGA	20	20	45.0	45.0	56.9	57.8	0.0	0.0	1.277647	114301	115482	1366-1366	1.000000	69.8
ndhH_p3	ndhH	3	TCCCAAAAGGAGGGTCAAAA	TTTGATGAGCCTCGCTTTGA	20	20	45.0	45.0	56.8	57.8	0.0	0.0	1.340108	114301	115482	1371-1371	1.000000	69.3
ndhH_p4	ndhH	4	AGGAGGGTCAAAATGGGTAGA	TTGATGAGCCTCGCTTTGAG	21	20	47.6	50.0	58.1	58.3	0.0	0.0	1.340198	114301	115482	1363-1363	1.000000	69.3
ndhH_p5	ndhH	5	AAAGGAGGGTCAAAATGGGT	TTGATGAGCCTCGCTTTGAG	20	20	45.0	50.0	56.9	58.3	0.0	0.0	1.363668	114301	115482	1365-1365	1.000000	69.1
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	CAGAACCCCTGTTTTCTTCT	ACGACCAGTCAGTTAACAGC	20	20	45.0	50.0	55.2	57.8	0.0	8.6	2.982252	115901	117431	1653-1653	1.000000	56.2
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	CAGAACCCCTGTTTTCTTCT	AGGATTCGAACCTACGACCA	20	20	45.0	50.0	55.2	58.2	0.0	15.0	2.988487	115901	117431	1666-1666	1.000000	56.2
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	CAGAACCCCTGTTTTCTTCT	GGATTCGAACCTACGACCAG	20	20	45.0	55.0	55.2	57.8	0.0	0.0	3.037162	115901	117431	1665-1665	1.000000	55.8
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	ACTTGAAATTCAACAGAACCCC	ACGACCAGTCAGTTAACAGC	22	20	40.9	50.0	57.1	57.8	4.0	8.6	3.087598	115901	117431	1666-1666	1.000000	55.4
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	ACTTGAAATTCAACAGAACCCC	AGGATTCGAACCTACGACCA	22	20	40.9	50.0	57.1	58.2	4.0	15.0	3.093833	115901	117431	1679-1679	1.000000	55.3
rrn16_p1	rrn16	1	CAGACGTAGCTGCCTTCTTT	TGGCGAACTCCAGGCTAATA	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.594386	123784	125275	1690-1690	1.000000	75.3
rrn16_p2	rrn16	2	CAGACGTAGCTGCCTTCTTT	GGCGAACTCCAGGCTAATAA	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.700883	123784	125275	1689-1689	1.000000	74.4
rrn16_p3	rrn16	3	TTTTGGGCGTGAAGAAGTGT	TGGCGAACTCCAGGCTAATA	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.750649	123784	125275	1672-1672	1.000000	74.0
rrn16_p4	rrn16	4	TTTTGGGCGTGAAGAAGTGT	GGCGAACTCCAGGCTAATAA	20	20	45.0	50.0	58.2	57.4	0.0	0.0	0.857146	123784	125275	1671-1671	1.000000	73.2
rrn16_p5	rrn16	5	GCGTGAAGAAGTGTCAAACC	TGGCGAACTCCAGGCTAATA	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.893230	123784	125275	1666-1666	1.000000	72.9
rps7_p1	rps7	1	TCTTGAACCTCTTTCACGCT	TCCGATCGAGATGTACGGAT	20	20	45.0	50.0	57.1	57.8	0.0	18.7	1.091120	128151	128621	546-546	1.000000	71.3
rps7_p2	rps7	2	TCTTTCGAGATCCACCCTACA	TCCGATCGAGATGTACGGAT	21	20	47.6	50.0	57.9	57.8	0.0	18.7	1.276344	128151	128621	627-627	1.000000	69.8
rps7_p3	rps7	3	TTCTTGAACCTCTTTCACGCT	TCCGATCGAGATGTACGGAT	21	20	42.9	50.0	57.8	57.8	0.0	18.7	1.374437	128151	128621	547-547	1.000000	69.0
rps7_p4	rps7	4	CTTGAACCTCTTTCACGCTC	TCCGATCGAGATGTACGGAT	20	20	50.0	50.0	56.8	57.8	0.0	18.7	1.420407	128151	128621	545-545	1.000000	68.6
rps7_p5	rps7	5	TCTTGAACCTCTTTCACGCTC	TCCGATCGAGATGTACGGAT	21	20	47.6	50.0	58.2	57.8	0.0	18.7	1.424783	128151	128621	546-546	1.000000	68.6
trnL-CAA-trnI-CAU_p1	trnL-CAA-trnI-CAU	1	AAATGGTAGACACGCGAGAC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	15.9	7.6	0.017031	131819	134202	2480-2485	1.000000	80.1
trnL-CAA-trnI-CAU_p2	trnL-CAA-trnI-CAU	2	TGAAATGGTAGACACGCGAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	15.9	7.6	0.018402	131819	134202	2482-2487	1.000000	80.0
trnL-CAA-trnI-CAU_p3	trnL-CAA-trnI-CAU	3	TGGTGAAATGGTAGACACGC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.2	58.0	0.0	7.6	0.205867	131819	134202	2485-2490	1.000000	78.5
trnL-CAA-trnI-CAU_p4	trnL-CAA-trnI-CAU	4	GAAATGGTAGACACGCGAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.7	58.0	15.9	7.6	0.282570	131819	134202	2481-2486	1.000000	77.9
trnL-CAA-trnI-CAU_p5	trnL-CAA-trnI-CAU	5	GTTCGAGTCCTCTTCAAGGC	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.3	58.0	0.0	7.6	0.293208	131819	134202	2432-2437	1.000000	77.9