Hotspot

rps15

Genome · 113631-113897 · 267 bp

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Pi 0.447

MarkerSeek score 76.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: Genome
length_bp: 267
variable_sites: 115
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACGGGCTCATAGGAGTAGAC	GCCATAGGTTTTCCCCGATT	55.0	50.0	58.0	57.9	1062 bp	0.5	59.3
rps15_p2	CACGGGCTCATAGGAGTAGA	GCCATAGGTTTTCCCCGATT	55.0	50.0	58.0	57.9	1063 bp	0.5	59.3
rps15_p3	CGGGCTCATAGGAGTAGACA	GCCATAGGTTTTCCCCGATT	55.0	50.0	58.0	57.9	1061 bp	0.5	59.3
rps15_p4	TCACGGGCTCATAGGAGTAG	GCCATAGGTTTTCCCCGATT	55.0	50.0	58.0	57.9	1064 bp	0.5	59.3
rps15_p5	ACCTAGACGTCGTCGAGAAT	GCCATAGGTTTTCCCCGATT	50.0	50.0	58.0	57.9	966 bp	0.5	59.3