Hotspot

ndhI

Genome · 117520-118035 · 516 bp

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Pi 0.482

MarkerSeek score 81.00

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 516
variable_sites: 228
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TGGGTGGTTGGAATCTCTCT	ACCAACGAAATAGCCCCATC	50.0	50.0	58.0	57.9	1589 bp	0.5	59.2
ndhI_p2	TGGGTGGTTGGAATCTCTCT	TAATACCACTCCGAGACCCC	50.0	55.0	58.0	57.9	1365 bp	0.5	59.0
ndhI_p3	TGGGTGGTTGGAATCTCTCT	AAGACCTGACCCCAGAAAGA	50.0	50.0	58.0	57.9	1338 bp	0.5	58.7
ndhI_p4	TGGGTGGTTGGAATCTCTCT	ATTCTGGCCCACTCATGAAC	50.0	50.0	58.0	57.9	1537 bp	0.5	58.6
ndhI_p5	TGGGTGGTTGGAATCTCTCT	CTGGCCCACTCATGAACATT	50.0	50.0	58.0	58.2	1534 bp	0.5	58.4