Hotspot

ndhG

Genome · 118403-118933 · 531 bp

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Pi 0.474

MarkerSeek score 79.00

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 531
variable_sites: 211
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGATCACATCGGAGCGTTTT	AGTCCAATAGCAGCTTCAGC	45.0	50.0	57.9	58.0	1760 bp	0.5	59.0
ndhG_p2	TTGATCACATCGGAGCGTTT	AGTCCAATAGCAGCTTCAGC	45.0	50.0	57.9	58.0	1761 bp	0.5	59.0
ndhG_p3	AAAAGAATGCGATGGGTCCA	AGTCCAATAGCAGCTTCAGC	45.0	50.0	57.8	58.0	1171 bp	0.5	58.0
ndhG_p4	AAAGAATGCGATGGGTCCAA	AGTCCAATAGCAGCTTCAGC	45.0	50.0	57.8	58.0	1170 bp	0.5	58.0
ndhG_p5	TCAAATTGCTCTGGGTCGTT	AGTCCAATAGCAGCTTCAGC	45.0	50.0	57.7	58.0	1486 bp	0.5	57.6