Hotspot

ndhA

Genome · 115201-117387 · 2187 bp

Back to Tacinga

Pi 0.447

MarkerSeek score 78.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 2187
variable_sites: 790
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGTTTATCGGGACCAATGCT	CGTACCGCTTGTTGACCATA	50.0	50.0	57.9	58.0	2901 bp	0.5	59.6
ndhA_p2	TGGGAAGTCCAATGGCAAAA	CGTACCGCTTGTTGACCATA	45.0	50.0	57.9	58.0	2814 bp	0.5	59.2
ndhA_p3	TTCGTTCCCCGGGTTTTATC	CGTACCGCTTGTTGACCATA	50.0	50.0	57.9	58.0	2492 bp	0.5	59.1
ndhA_p4	AGTAAAAAGCCATCCCCTGC	CGTACCGCTTGTTGACCATA	50.0	50.0	58.1	58.0	2619 bp	0.5	58.8
ndhA_p5	GTAAAAAGCCATCCCCTGCT	CGTACCGCTTGTTGACCATA	50.0	50.0	58.1	58.0	2618 bp	0.5	58.8