Hotspot

ndhD-ccsA

Genome · 121689-121907 · 219 bp

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Pi 0.449

MarkerSeek score 76.10

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: Genome
length_bp: 219
variable_sites: 89
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	GGCATGAGTGGTTTCATTGC	ATGCAGCTCTTTTATGCGGA	50.0	45.0	58.0	58.0	1097 bp	0.5	59.7
ndhD-ccsA_p2	GGCATGAGTGGTTTCATTGC	TGCAGCTCTTTTATGCGGAT	50.0	45.0	58.0	58.0	1096 bp	0.5	59.7
ndhD-ccsA_p3	GTTTACCCGGATTTCGCTCT	ATGCAGCTCTTTTATGCGGA	50.0	45.0	58.0	58.0	836 bp	0.5	59.5
ndhD-ccsA_p4	GTTTACCCGGATTTCGCTCT	TGCAGCTCTTTTATGCGGAT	50.0	45.0	58.0	58.0	835 bp	0.5	59.5
ndhD-ccsA_p5	GTATTGGCGTTTACCCGGAT	TGCAGCTCTTTTATGCGGAT	50.0	45.0	58.0	58.0	843 bp	0.5	59.3