Hotspot

ndhE-psaC

Genome · 119492-119817 · 326 bp

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Pi 0.456

MarkerSeek score 77.50

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: Genome
length_bp: 326
variable_sites: 139
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GCTGAAGCTGCTATTGGACT	TTTGGCCACTGTACATTGCT	50.0	45.0	58.0	58.0	1163 bp	0.5	59.7
ndhE-psaC_p2	GCTGAAGCTGCTATTGGACT	GCCTTACATCCATCCCAAGG	50.0	55.0	58.0	58.0	526 bp	0.5	59.6
ndhE-psaC_p3	GCTGAAGCTGCTATTGGACT	AGAATTTCGAGTAACCGGCC	50.0	50.0	58.0	58.0	1120 bp	0.5	59.6
ndhE-psaC_p4	GCTGAAGCTGCTATTGGACT	AACCTGCAAATAGGGGCAAA	50.0	45.0	58.0	58.0	842 bp	0.5	59.5
ndhE-psaC_p5	GCTGAAGCTGCTATTGGACT	ACCTGCAAATAGGGGCAAAA	50.0	45.0	58.0	58.0	841 bp	0.5	59.5