Hotspot

ndhD

SSC · 123269-124603 · 1335 bp

Pi 0.0292

MarkerSeek score 61.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1335
variable_sites: 39
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AATCCGGCACTTGTAGCTTC	AACCACCGATTCATCTGGTG	50.0	50.0	58.3	57.9	2360-2452 bp	1.0	80.3
ndhD_p2	GAATCCGGCACTTGTAGCTT	AACCACCGATTCATCTGGTG	50.0	50.0	58.3	57.9	2361-2453 bp	1.0	80.3
ndhD_p3	AATCCGGCACTTGTAGCTTC	GAGCACGGGTTTTCTGGTTA	50.0	50.0	58.3	58.1	1877-1914 bp	1.0	80.1
ndhD_p4	GAATCCGGCACTTGTAGCTT	GAGCACGGGTTTTCTGGTTA	50.0	50.0	58.3	58.1	1878-1915 bp	1.0	80.1
ndhD_p5	TTTCAAGGTACGAATCCGGC	GAGCACGGGTTTTCTGGTTA	50.0	50.0	58.3	58.1	1889-1926 bp	1.0	80.0