Hotspot

psaC-ndhE

SSC · 125147-125582 · 436 bp

Back to Tacca

Pi 0.0769
MarkerSeek score 80.80
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
436
variable_sites
33
pi
0.0769
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CCAATACATCTGTGGGGCAA AGCTATTGTTTCGTCGATCCA 50.0 42.9 57.9 57.5 588-682 bp 1.0 71.7
psaC-ndhE_p2 ACATCCATCCCACGGTATCA AGCTATTGTTTCGTCGATCCA 50.0 42.9 58.2 57.5 611-705 bp 1.0 71.1
psaC-ndhE_p3 AATACATCTGTGGGGCAAGC AGCTATTGTTTCGTCGATCCA 50.0 42.9 58.2 57.5 586-680 bp 1.0 71.0
psaC-ndhE_p4 AGCTTTACATCCATCCCACG AGCTATTGTTTCGTCGATCCA 50.0 42.9 57.7 57.5 617-711 bp 1.0 70.0
psaC-ndhE_p5 TTCCAATACATCTGTGGGGC AGCTATTGTTTCGTCGATCCA 50.0 42.9 57.6 57.5 590-684 bp 1.0 69.4