Hotspot

psbT-psbN

LSC · 74157-74222 · 66 bp

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Pi 0.133
MarkerSeek score 77.50
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
66
variable_sites
8
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 ACGGAATTGTTAGAGCCGAC CGCAAATAAAGCCATTGCCA 50.0 45.0 58.0 58.0 1150-1155 bp 1.0 82.3
psbT-psbN_p2 ATTGTTAGAGCCGACGTTCC CGCAAATAAAGCCATTGCCA 50.0 45.0 58.0 58.0 1145-1150 bp 1.0 82.3
psbT-psbN_p3 GGAATTGTTAGAGCCGACGT CGCAAATAAAGCCATTGCCA 50.0 45.0 58.0 58.0 1148-1153 bp 1.0 82.3
psbT-psbN_p4 ATTGTTAGAGCCGACGTTCC ACCGCAAATAAAGCCATTGC 50.0 45.0 58.0 58.0 1147-1152 bp 1.0 82.2
psbT-psbN_p5 GGAATTGTTAGAGCCGACGT ACCGCAAATAAAGCCATTGC 50.0 45.0 58.0 58.0 1150-1155 bp 1.0 82.2