Hotspot

petA-psbJ

LSC · 63017-63868 · 852 bp

Back to Tacca

Pi 0.0473
MarkerSeek score 79.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
852
variable_sites
37
pi
0.0473
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTTATTTTGGCACA GGTTCATATTCCGGGTTGGG 42.9 55.0 57.4 58.3 970-1275 bp 1.0 71.4
petA-psbJ_p2 TGGCATCTGTTATTTTGGCAC GGTTCATATTCCGGGTTGGG 42.9 55.0 57.4 58.3 971-1276 bp 1.0 71.4
petA-psbJ_p3 GGCATCTGTTATTTTGGCACA CATATTCCGGGTTGGGTTCA 42.9 50.0 57.4 57.6 966-1271 bp 1.0 70.5
petA-psbJ_p4 TGGCATCTGTTATTTTGGCAC CATATTCCGGGTTGGGTTCA 42.9 50.0 57.4 57.6 967-1272 bp 1.0 70.5
petA-psbJ_p5 GGCATCTGTTATTTTGGCACA GTTCATATTCCGGGTTGGGT 42.9 50.0 57.4 57.6 969-1274 bp 1.0 70.5