Hotspot

ycf4-cemA

LSC · 60322-61116 · 795 bp

Pi 0.0487

MarkerSeek score 77.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 37
pi: 0.0487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCACGT	TGTGTCTCGGGGTACTCTTT	45.0	50.0	58.1	58.0	1056-1072 bp	1.0	85.2
ycf4-cemA_p2	TTTCGTTGGGGATTTCCTGG	TGTGTCTCGGGGTACTCTTT	50.0	50.0	58.1	58.0	1260-1276 bp	1.0	84.7
ycf4-cemA_p3	CGGTATTTCCATCCCAGCAA	TGTGTCTCGGGGTACTCTTT	50.0	50.0	57.9	58.0	1424-1440 bp	1.0	84.6
ycf4-cemA_p4	AGGGTCTTTATCCTCGTCGT	TGTGTCTCGGGGTACTCTTT	50.0	50.0	57.9	58.0	1172-1188 bp	1.0	84.6
ycf4-cemA_p5	TGTCTTTCTACGGAATCGCG	TGTGTCTCGGGGTACTCTTT	50.0	50.0	58.1	58.0	1376-1392 bp	1.0	84.3