Hotspot

ndhC-trnV-UAC

LSC · 50604-51398 · 795 bp

Back to Tacca

Pi 0.0835
MarkerSeek score 80.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
795
variable_sites
66
pi
0.0835
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGTG GGTTCGAATCCGTATAGCCC 37.5 55.0 57.4 57.9 853-936 bp 1.0 49.3
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGTG CGGTTCGAATCCGTATAGCC 37.5 55.0 57.4 58.3 854-937 bp 1.0 47.4
ndhC-trnV-UAC_p3 GCCCAGAAAATATCATATTCGTGA GGTTCGAATCCGTATAGCCC 37.5 55.0 57.2 57.9 852-935 bp 1.0 47.3
ndhC-trnV-UAC_p4 TGCCCAGAAAATATCATATTCGT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.1 57.9 853-936 bp 1.0 46.7
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCATATTCGTG AGCCCAATGCAACAAAATGC 37.5 45.0 57.4 58.5 946-1029 bp 1.0 45.8