Hotspot

trnT-UGU-trnL-UAA

LSC · 46158-47096 · 939 bp

Back to Tacca

Pi 0.0407
MarkerSeek score 77.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
939
variable_sites
37
pi
0.0407
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 968-989 bp 1.0 84.4
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 55.0 50.0 58.2 58.0 999-1020 bp 1.0 84.0
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 976-997 bp 1.0 83.8
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CAAAGTCCGTAGCGTCTACC 55.0 55.0 57.9 58.1 985-1006 bp 1.0 83.6
trnT-UGU-trnL-UAA_p5 GAGTCGAACCGATGACCATC TAGCGTCTACCAATTTCGCC 55.0 50.0 58.2 58.1 1007-1028 bp 1.0 83.4