Hotspot

rpoC1-rpoB

LSC · 22241-22245 · 5 bp

Back to Tacca

Pi 0.2
MarkerSeek score 78.80
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
LSC
length_bp
5
variable_sites
1
pi
0.2
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 GTTGAGGAGAAACTGGTCCA TTGCTCGTTCGAGAACTACG 50.0 50.0 57.1 58.0 174 bp 1.0 72.7
rpoC1-rpoB_p2 TGTTGAGGAGAAACTGGTCC TTGCTCGTTCGAGAACTACG 50.0 50.0 57.1 58.0 175 bp 1.0 72.7
rpoC1-rpoB_p3 GTTGAGGAGAAACTGGTCCA TACGATCCTTGGCTCTGGAA 50.0 50.0 57.1 58.1 158 bp 1.0 71.9
rpoC1-rpoB_p4 TGTTGAGGAGAAACTGGTCC TACGATCCTTGGCTCTGGAA 50.0 50.0 57.1 58.1 159 bp 1.0 71.9
rpoC1-rpoB_p5 GTTGAGGAGAAACTGGTCCA CGAGAACTACGATCCTTGGC 50.0 55.0 57.1 58.2 165 bp 1.0 71.8