Hotspot

atpF-atpH

LSC · 11688-12113 · 426 bp

Back to Tacca

Pi 0.0851
MarkerSeek score 76.30
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
426
variable_sites
36
pi
0.0851
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GAGGAAACAAAAGAATCGGT AATTTACGGGCTGGTCGTG 40.0 52.6 53.8 58.2 513-518 bp 1.0 46.1
atpF-atpH_p2 GAGGAAACAAAAGAATCGGT AACAATTTACGGGCTGGTCG 40.0 50.0 53.8 58.8 516-521 bp 1.0 46.0
atpF-atpH_p3 GAGGAAACAAAAGAATCGGTTACA AACAATTTACGGGCTGGTCG 37.5 50.0 57.7 58.8 516-521 bp 1.0 46.0
atpF-atpH_p4 GAGGAAACAAAAGAATCGGTT AACAATTTACGGGCTGGTCG 38.1 50.0 54.7 58.8 516-521 bp 1.0 46.0
atpF-atpH_p5 AGGAAACAAAAGAATCGGTTACA AACAATTTACGGGCTGGTCG 34.8 50.0 56.6 58.8 515-520 bp 1.0 46.0