Hotspot

trnK-UUU-trnQ-UUG

LSC · 3969-5125 · 1157 bp

Back to Tacca

Pi 0.0622
MarkerSeek score 81.40
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1157
variable_sites
70
pi
0.0622
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1215-2317 bp 1.0 82.7
trnK-UUU-trnQ-UUG_p2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1214-2316 bp 1.0 82.7
trnK-UUU-trnQ-UUG_p3 TCGTTGCCTCATCCAAATGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1258-2360 bp 1.0 82.3
trnK-UUU-trnQ-UUG_p4 CGTTGCCTCATCCAAATGTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1257-2359 bp 1.0 81.8
trnK-UUU-trnQ-UUG_p5 AAGCCGAGTACTCTACCGTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1218-2320 bp 1.0 81.6