Hotspot

ycf1

SSC · 131414-137014 · 5601 bp

Back to Tabernaemontana

Pi 0.00803

MarkerSeek score 44.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 45
pi: 0.00803
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACGAGTCTCCTTTATTTTCA	GGAAAAACTGGTTAAAGGGACA	36.4	40.9	54.9	56.7	281 bp	1.0	41.4
ycf1_p2	AGACGAGTCTCCTTTATTTTCA	TGGAAAAACTGGTTAAAGGGAC	36.4	40.9	54.9	56.7	282 bp	1.0	41.4
ycf1_p3	AGACGAGTCTCCTTTATTTTCA	TGGAAAAACTGGTTAAAGGGACA	36.4	39.1	54.9	58.4	282 bp	1.0	41.4
ycf1_p4	AGACGAGTCTCCTTTATTTTCAT	GGAAAAACTGGTTAAAGGGACA	34.8	40.9	55.4	56.7	281 bp	1.0	41.4
ycf1_p5	AGACGAGTCTCCTTTATTTTCAT	TGGAAAAACTGGTTAAAGGGAC	34.8	40.9	55.4	56.7	282 bp	1.0	41.4