Hotspot

clpP

LSC · 77806-79832 · 2027 bp

Back to Tabernaemontana

Pi 0.00692

MarkerSeek score 48.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2027
variable_sites: 14
pi: 0.00692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACATGTTCCTCGACGTTGAG	TTCGTTACAGTCCCTCCTGT	50.0	50.0	57.9	58.0	2975-2978 bp	1.0	80.0
clpP_p2	ACATGTTCCTCGACGTTGAG	CATAGAGAGCCATCGAACCG	50.0	55.0	57.9	57.9	2836-2839 bp	1.0	79.7
clpP_p3	ACATGTTCCTCGACGTTGAG	ACGAACATACCCTGTCTCCA	50.0	50.0	57.9	58.1	2900-2903 bp	1.0	79.6
clpP_p4	ACATGTTCCTCGACGTTGAG	GTCCCTCCTGTGATACTCCA	50.0	55.0	57.9	57.8	2966-2969 bp	1.0	78.8
clpP_p5	ACATGTTCCTCGACGTTGAG	AAGAACAGGGTCAGAAGGGT	50.0	50.0	57.9	58.2	2871-2874 bp	1.0	78.7