Hotspot

atpF

LSC · 11926-13205 · 1280 bp

Back to Tabernaemontana

Pi 0.00393

MarkerSeek score 61.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1280
variable_sites: 5
pi: 0.00393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCGTCAGCTCGAATGGTT	AAGAGGGGCGAAGTGATAGA	45.0	50.0	57.8	57.8	1438-1440 bp	1.0	78.1
atpF_p2	TTTCGTCAGCTCGAATGGTT	AGAGGGGCGAAGTGATAGAA	45.0	50.0	57.8	57.8	1437-1439 bp	1.0	78.1
atpF_p3	TCGTCAGCTCGAATGGTTAC	AAGAGGGGCGAAGTGATAGA	50.0	50.0	57.7	57.8	1436-1438 bp	1.0	77.3
atpF_p4	TCGTCAGCTCGAATGGTTAC	AGAGGGGCGAAGTGATAGAA	50.0	50.0	57.7	57.8	1435-1437 bp	1.0	77.3
atpF_p5	GTCAGCTCGAATGGTTACCA	AGAGGGGCGAAGTGATAGAA	50.0	50.0	57.6	57.8	1433-1435 bp	1.0	76.4