Hotspot

ndhF-rpl32

SSC · 120008-120741 · 734 bp

Back to Tabernaemontana

Pi 0.0124

MarkerSeek score 54.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 734
variable_sites: 9
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTATGAGCCACCCGTATTG	AAAGCCTTTAACGCTGTCCA	55.0	45.0	58.1	58.0	864-880 bp	1.0	80.2
ndhF-rpl32_p2	GGTATGAGCCACCCGTATTG	AAGCCTTTAACGCTGTCCAA	55.0	45.0	58.1	58.0	863-879 bp	1.0	80.2
ndhF-rpl32_p3	GGTATGAGCCACCCGTATTG	CGCTGTCCAATATCCTTCCC	55.0	55.0	58.1	58.1	853-869 bp	1.0	79.5
ndhF-rpl32_p4	GGTATGAGCCACCCGTATTG	AGCCTTTAACGCTGTCCAAT	55.0	45.0	58.1	57.8	862-878 bp	1.0	78.8
ndhF-rpl32_p5	GGTATGAGCCACCCGTATTG	ACGCTGTCCAATATCCTTCC	55.0	50.0	58.1	57.4	854-870 bp	1.0	75.4