Hotspot

ycf2-trnL-CAA

IRb · 101077-101756 · 680 bp

Back to Tabernaemontana

Pi 0.00735

MarkerSeek score 63.00

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 680
variable_sites: 5
pi: 0.00735
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	789 bp	1.0	78.6
ycf2-trnL-CAA_p2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	788 bp	1.0	78.6
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	791 bp	1.0	78.6
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	790 bp	1.0	78.6
ycf2-trnL-CAA_p5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	792 bp	1.0	78.6