Hotspot

psbZ-trnG-GCC

LSC · 37379-43598 · 6220 bp

Back to Tabernaemontana

Pi 0.0143

MarkerSeek score 52.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 6220
variable_sites: 39
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.44
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTCGAAGAAAGGTAAGCGCT	CCCTTAATCCATGCCCTTCC	50.0	55.0	58.0	58.0	842-849 bp	1.0	82.4
psbZ-trnG-GCC_p2	TCGAAGAAAGGTAAGCGCTC	CCCTTAATCCATGCCCTTCC	50.0	55.0	58.0	58.0	841-848 bp	1.0	82.4
psbZ-trnG-GCC_p3	CAAAGGGAGATGGATCCAGC	CCCTTAATCCATGCCCTTCC	55.0	55.0	58.0	58.0	1049 bp	0.5	59.8
psbZ-trnG-GCC_p4	GCAAAGGGAGATGGATCCAG	CCCTTAATCCATGCCCTTCC	55.0	55.0	58.0	58.0	1050 bp	0.5	59.8
psbZ-trnG-GCC_p5	AGGGAGATGGATCCAGCTAC	CCCTTAATCCATGCCCTTCC	55.0	55.0	58.0	58.0	1046 bp	0.5	59.8