Hotspot

rpoC1-rpoB

LSC · 23909-23913 · 5 bp

Back to Tabernaemontana

Pi 0.2

MarkerSeek score 87.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 5
variable_sites: 1
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	CAGGATTTTAGTGGCCCAGG	CGAGGATGCTCCAGAATCTT	55.0	50.0	58.2	57.1	228 bp	1.0	71.2
rpoC1-rpoB_p2	CAGGATTTTAGTGGCCCAGG	CCGAGGATGCTCCAGAATCT	55.0	55.0	58.2	59.0	229 bp	1.0	70.9
rpoC1-rpoB_p3	AGGCGCTTATTTGTTGAGGA	CGAGGATGCTCCAGAATCTT	45.0	50.0	57.5	57.1	211 bp	1.0	69.2
rpoC1-rpoB_p4	ACCTCTCCATTAGGCAGGAT	CGAGGATGCTCCAGAATCTT	50.0	50.0	57.5	57.1	242 bp	1.0	69.0
rpoC1-rpoB_p5	CAGGATTTTAGTGGCCCAGG	CGAGGATGCTCCAGAATCTTT	55.0	47.6	58.2	57.8	228 bp	1.0	69.0