Hotspot

ycf1

SSC · 128192-133816 · 5625 bp

Back to Syzygium

Pi 0.00904

MarkerSeek score 72.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 1071
pi: 0.00904
unique_haplotype_count: 162
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTGACCCTTCCACGACAG	AGAATATCTTTTGCGTACCCCC	50.0	45.5	57.8	58.2	310-316 bp	0.9375	66.6
ycf1_p2	ATTTGACCCTTCCACGACAG	GAATATCTTTTGCGTACCCCC	50.0	47.6	57.8	56.7	309-315 bp	0.9375	66.0
ycf1_p3	ATTTGACCCTTCCACGACAG	AATATCTTTTGCGTACCCCC	50.0	45.0	57.8	55.5	308-314 bp	0.9375	63.9
ycf1_p4	AATTTGACCCTTCCACGACA	AGAATATCTTTTGCGTACCCCC	45.0	45.5	57.4	58.2	311-317 bp	0.9375	62.8
ycf1_p5	AATTTGACCCTTCCACGACA	GAATATCTTTTGCGTACCCCC	45.0	47.6	57.4	56.7	310-316 bp	0.9375	62.2