Hotspot

rpl22

LSC · 87371-87853 · 483 bp

Back to Syzygium

Pi 0.0115

MarkerSeek score 50.50

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 483
variable_sites: 100
pi: 0.0115
unique_haplotype_count: 105
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTTTGGCTTGTGCAAACCAA	TGATCATTAGTAAGAGGTGAACC	40.0	39.1	57.5	55.5	571-599 bp	0.960227	46.8
rpl22_p2	TTTTTGGCTTGTGCAAACCA	TGATCATTAGTAAGAGGTGAACC	40.0	39.1	57.5	55.5	572-600 bp	0.960227	46.8
rpl22_p3	TTGGCTTGTGCAAACCAAAT	TGATCATTAGTAAGAGGTGAACC	40.0	39.1	57.3	55.5	569-597 bp	0.960227	46.8
rpl22_p4	GATGACCTTGGGTTGTACCA	TGATCATTAGTAAGAGGTGAACC	50.0	39.1	57.1	55.5	544-572 bp	0.960227	46.8
rpl22_p5	TGATGACCTTGGGTTGTACC	TGATCATTAGTAAGAGGTGAACC	50.0	39.1	57.1	55.5	545-573 bp	0.960227	46.8