Hotspot

rpl32-trnL-UAG

SSC · 117592-118326 · 735 bp

Back to Syzygium

Pi 0.0128
MarkerSeek score 72.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
735
variable_sites
166
pi
0.0128
unique_haplotype_count
158
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGAAGTCTCTTTCTACCGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 678-861 bp 0.994318 91.0
rpl32-trnL-UAG_p2 AGGTATATTGGGCAGCGTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 713-896 bp 0.971591 90.5
rpl32-trnL-UAG_p3 AGGTATATTGGGCAGCGTTG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 714-897 bp 0.971591 90.5
rpl32-trnL-UAG_p4 GGTATATTGGGCAGCGTTGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 712-895 bp 0.971591 90.5
rpl32-trnL-UAG_p5 GGTATATTGGGCAGCGTTGA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 713-896 bp 0.971591 90.5