Hotspot

ndhF-rpl32

SSC · 116480-117417 · 938 bp

Back to Syzygium

Pi 0.0162
MarkerSeek score 67.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
938
variable_sites
334
pi
0.0162
unique_haplotype_count
150
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAGGGG TCCAAATGTTTTTACGAATACG 55.0 31.8 57.9 53.5 581-1080 bp 0.994318 51.8
ndhF-rpl32_p2 TGGAAGTGGAATGAAGGGGA TCCAAATGTTTTTACGAATACG 50.0 31.8 58.2 53.5 580-1079 bp 0.994318 51.8
ndhF-rpl32_p3 CTGGAAGTGGAATGAAGGGG TTCCAAATGTTTTTACGAATACG 55.0 30.4 57.9 54.3 582-1081 bp 0.994318 51.8
ndhF-rpl32_p4 TGGAAGTGGAATGAAGGGGA TTCCAAATGTTTTTACGAATACG 50.0 30.4 58.2 54.3 581-1080 bp 0.994318 51.8
ndhF-rpl32_p5 CTGGAAGTGGAATGAAGGGG TTTCCAAATGTTTTTACGAATACG 55.0 29.2 57.9 55.0 583-1082 bp 0.994318 51.8